Python实现GB格式序列文件转换Fasta格式文件

目录

• GB格式文件和FASTA文件介绍
• 处理步骤
• Python脚本代码如下：
• 运行情况

GB格式文件和FASTA文件介绍

在分子生物学中 我们会有将GB格式序列文件 转换成 Fasta格式文件的需求，这里我们利用python脚本来解决这个问题。

gb格式文件是GenBank的文件，用来保存序列的详细信息。包含一个gene的名称，编号，发现者，参考文献，外显子位置，编码区序列，蛋白序列等等信息。

例如：

LOCUS       NM_213806                849 bp    mRNA    linear   MAM 24-SEP-2019
DEFINITION  Sus scrofa Fas ligand (TNF superfamily, member 6) (FASLG), mRNA.
ACCESSION   NM_213806
VERSION     NM_213806.1
KEYWORDS    RefSeq.
SOURCE      Sus scrofa (pig)
  ORGANISM  Sus scrofa
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Suina; Suidae;
            Sus.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Lin F, Fu YH, Han J, Shen M, Du CW, Li R, Ma XS and Liu HL.
  TITLE     Changes in the expression of Fox O1 and death ligand genes during
            follicular atresia in porcine ovary
  JOURNAL   Genet. Mol. Res. 13 (3), 6638-6645 (2014)
   PUBMED   25177944
  REMARK    GeneRIF: Data suggest forkhead box protein O1 (FoxO1) involvement
            in the regulation of TNF-related apoptosis-inducing ligand TRAIL
            and Fas ligand FasL expression during follicular atresia.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  AUTHORS   Xie GH, Wang SJ, Wang Y, Zhang Y, Zhang HZ, Jin S, Wang QF, Liu ZC
            and Ge HL.
  TITLE     Fas Ligand gene transfer enhances the survival of tissue-engineered
            chondrocyte allografts in mini-pigs
  JOURNAL   Transpl. Immunol. 19 (2), 145-151 (2008)
   PUBMED   18503890
  REMARK    GeneRIF: the result indicates that the expression of FasL by
            chondrocytes is capable of inducing apoptosis of activated T cells
REFERENCE   3  (bases 1 to 849)
  AUTHORS   Chang HW, Jeng CR, Lin CM, Liu JJ, Chang CC, Tsai YC, Chia MY and
            Pang VF.
  TITLE     The involvement of Fas/FasL interaction in porcine circovirus type
            2 and porcine reproductive and respiratory syndrome virus
            co-inoculation-associated lymphocyte apoptosis in vitro
  JOURNAL   Vet. Microbiol. 122 (1-2), 72-82 (2007)
   PUBMED   17321702
  REMARK    GeneRIF: The expression of FAS and FAS ligand in splenic
            macrophages co-infected with porcine circovirus 2 and porcine
            reproductive and respiratory syndrome virus is reported
REFERENCE   4  (bases 1 to 849)
  AUTHORS   Tayade C, Black GP, Fang Y and Croy BA.
  TITLE     Differential gene expression in endometrium, endometrial
            lymphocytes, and trophoblasts during successful and abortive embryo
            implantation
  JOURNAL   J. Immunol. 176 (1), 148-156 (2006)
   PUBMED   16365405
REFERENCE   5  (bases 1 to 849)
  AUTHORS   Bai L, Maedler K, Donath M and Tuch BE.
  TITLE     Expression of Fas but not Fas ligand on fetal pig beta cells
  JOURNAL   Xenotransplantation 11 (5), 426-435 (2004)
   PUBMED   15303979
  REMARK    GeneRIF: FasL was not detected on fetal pig pancreatic cells but
            could be induced on both beta and non-beta cells when the cells
            were treated with IL1beta.
            Erratum:[Xenotransplantation. 2016 Mar;23(2):171-2. PMID: 27106874]
REFERENCE   6  (bases 1 to 849)
  AUTHORS   Tsuyuki S, Kono M and Bloom ET.
  TITLE     Cloning and potential utility of porcine Fas ligand: overexpression
            in porcine endothelial cells protects them from attack by human
            cytolytic cells
  JOURNAL   Xenotransplantation 9 (6), 410-421 (2002)
   PUBMED   12371937
REFERENCE   7  (bases 1 to 849)
  AUTHORS   Motegi-Ishiyama Y, Nakajima Y, Hoka S and Takagaki Y.
  TITLE     Porcine Fas-ligand gene: genomic sequence analysis and comparison
            with human gene
  JOURNAL   Mol. Immunol. 38 (8), 581-586 (2002)
   PUBMED   11792426
REFERENCE   8  (bases 1 to 849)
  AUTHORS   Muneta Y, Shimoji Y, Inumaru S and Mori Y.
  TITLE     Molecular cloning, characterization, and expression of porcine Fas
            ligand (CD95 ligand)
  JOURNAL   J. Interferon Cytokine Res. 21 (5), 305-312 (2001)
   PUBMED   11429161
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AB027297.1.
            ##Evidence-Data-START##
            Transcript exon combination :: AB027297.1, AF397407.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01893940, SAMN01915393
                                           [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Sus scrofa"
                     /mol_type="mRNA"
                     /db_xref="taxon:9823"
                     /chromosome="9"
                     /map="9"
     gene            1..849
                     /gene="FASLG"
                     /gene_synonym="CD95-L; FASL; TNFSF6"
                     /note="Fas ligand (TNF superfamily, member 6)"
                     /db_xref="GeneID:396726"
     CDS             1..849
                     /gene="FASLG"
                     /gene_synonym="CD95-L; FASL; TNFSF6"
                     /note="CD95 ligand; tumor necrosis factor (ligand)
                     superfamily, member 6; fas antigen ligand"
                     /codon_start=1
                     /product="tumor necrosis factor ligand superfamily member
                     6"
                     /protein_id="NP_998971.1"
                     /db_xref="GeneID:396726"
                     /translation="MQQPFNYPYPQIFWVDSSATSPWASPGSVFPCPASVPGRPGQRR
                     PPPPPPPPPPPPTLLPSRPLPPLPPPSLKKKRDHNAGLCLLVMFFMVLVALVGLGLGM
                     FQLFHLQKELTELRESASQRHTESSLEKQIGHPNLPSEKKELRKVAHLTGKPNSRSIP
                     LEWEDTYGIALVSGVKYMKGSLVINDTGLYFVYSKVYFRGQYCNNQPLSHKVYTRNSR
                     YPQDLVLMEGKMMNYCTTGQMWARSSYLGAVFNLTSADHLYVNVSELSLVNFEESKTF
                     FGLYKL"
     mat_peptide     1..390
                     /gene="FASLG"
                     /gene_synonym="CD95-L; FASL; TNFSF6"
                     /product="ADAM10-processed FasL form. {ECO:0000250}"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9BEA8.1)"
     mat_peptide     1..249
                     /gene="FASLG"
                     /gene_synonym="CD95-L; FASL; TNFSF6"
                     /product="FasL intracellular domain. {ECO:0000250}"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9BEA8.1)"
     misc_feature    244..249
                     /gene="FASLG"
                     /gene_synonym="CD95-L; FASL; TNFSF6"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Cleavage, by SPPL2A. {ECO:0000250}; propagated from
                     UniProtKB/Swiss-Prot (Q9BEA8.1); cleavage site"
     misc_feature    247..309
                     /gene="FASLG"
                     /gene_synonym="CD95-L; FASL; TNFSF6"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9BEA8.1);
                     transmembrane region"
     misc_feature    388..393
                     /gene="FASLG"
                     /gene_synonym="CD95-L; FASL; TNFSF6"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Cleavage, by ADAM10. {ECO:0000250}; propagated from
                     UniProtKB/Swiss-Prot (Q9BEA8.1); cleavage site"
     mat_peptide     391..846
                     /gene="FASLG"
                     /gene_synonym="CD95-L; FASL; TNFSF6"
                     /product="Tumor necrosis factor ligand superfamily member
                     6, soluble form. {ECO:0000250}"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9BEA8.1)"
     misc_feature    553..555
                     /gene="FASLG"
                     /gene_synonym="CD95-L; FASL; TNFSF6"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N-linked (GlcNAc...) asparagine. {ECO:0000255};
                     propagated from UniProtKB/Swiss-Prot (Q9BEA8.1);
                     glycosylation site"
     misc_feature    751..753
                     /gene="FASLG"
                     /gene_synonym="CD95-L; FASL; TNFSF6"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N-linked (GlcNAc...) asparagine. {ECO:0000255};
                     propagated from UniProtKB/Swiss-Prot (Q9BEA8.1);
                     glycosylation site"
     misc_feature    781..783
                     /gene="FASLG"
                     /gene_synonym="CD95-L; FASL; TNFSF6"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N-linked (GlcNAc...) asparagine. {ECO:0000255};
                     propagated from UniProtKB/Swiss-Prot (Q9BEA8.1);
                     glycosylation site"
     exon            1..351
                     /gene="FASLG"
                     /gene_synonym="CD95-L; FASL; TNFSF6"
                     /inference="alignment:Splign:2.1.0"
     exon            352..397
                     /gene="FASLG"
                     /gene_synonym="CD95-L; FASL; TNFSF6"
                     /inference="alignment:Splign:2.1.0"
     exon            398..454
                     /gene="FASLG"
                     /gene_synonym="CD95-L; FASL; TNFSF6"
                     /inference="alignment:Splign:2.1.0"
     exon            455..849
                     /gene="FASLG"
                     /gene_synonym="CD95-L; FASL; TNFSF6"
                     /inference="alignment:Splign:2.1.0"
ORIGIN
        1 atgcagcagc ccttcaatta cccatacccc caaatcttct gggtggacag cagtgctacc
       61 tctccctggg cctccccagg ctcagtcttc ccctgtccag cttctgtgcc aggaaggcca
      121 gggcaaagga ggccaccacc accaccgccg ccaccgccac caccaccaac actcctgcca
      181 tcaagaccgc tgcctccact gccaccgcca tctctgaaga agaagaggga ccacaatgca
      241 ggcctgtgtc tccttgtgat gttcttcatg gttctggtgg ccctggttgg attggggctg
      301 gggatgtttc agctcttcca cctacagaag gagctgactg aactcagaga gtctgccagc
      361 caaaggcata cagaatcatc tttggagaag caaataggtc accccaatct accctctgag
      421 aaaaaggagc tgagaaaagt ggcccactta acaggcaagc ctaactcaag atccatccct
      481 ctggaatggg aagacaccta tggaattgcc ttggtctctg gggtgaagta tatgaagggc
      541 agccttgtga tcaatgacac tgggctgtat tttgtgtatt ccaaagtgta cttccggggt
      601 cagtactgca acaaccagcc cctgagtcac aaggtataca caaggaactc taggtatccc
      661 caggacctgg tgctgatgga gggaaagatg atgaactatt gcactactgg ccaaatgtgg
      721 gcccgcagca gctacctggg ggctgtgttc aatctcacca gcgctgacca tttatatgtc
      781 aacgtatctg agctctctct ggtcaatttt gaggaatcta agacattttt tggcttatat
      841 aagctctga
//

fasta格式是一种基于文本用于表示核酸序列或多肽序列的格式。其中核酸或氨基酸均以单个字母来表示，且允许在序列前添加序列名及注释。该格式已成为生物信息学领域的一项标准。

例如：

>NM_213806
ATGCAGCAGCCCTTCAATTACCCATACCCCCAAATCTTCTGGGTGGACAGCAGTGCTACC
TCTCCCTGGGCCTCCCCAGGCTCAGTCTTCCCCTGTCCAGCTTCTGTGCCAGGAAGGCCA
GGGCAAAGGAGGCCACCACCACCACCGCCGCCACCGCCACCACCACCAACACTCCTGCCA
TCAAGACCGCTGCCTCCACTGCCACCGCCATCTCTGAAGAAGAAGAGGGACCACAATGCA
GGCCTGTGTCTCCTTGTGATGTTCTTCATGGTTCTGGTGGCCCTGGTTGGATTGGGGCTG
GGGATGTTTCAGCTCTTCCACCTACAGAAGGAGCTGACTGAACTCAGAGAGTCTGCCAGC
CAAAGGCATACAGAATCATCTTTGGAGAAGCAAATAGGTCACCCCAATCTACCCTCTGAG
AAAAAGGAGCTGAGAAAAGTGGCCCACTTAACAGGCAAGCCTAACTCAAGATCCATCCCT
CTGGAATGGGAAGACACCTATGGAATTGCCTTGGTCTCTGGGGTGAAGTATATGAAGGGC
AGCCTTGTGATCAATGACACTGGGCTGTATTTTGTGTATTCCAAAGTGTACTTCCGGGGT
CAGTACTGCAACAACCAGCCCCTGAGTCACAAGGTATACACAAGGAACTCTAGGTATCCC
CAGGACCTGGTGCTGATGGAGGGAAAGATGATGAACTATTGCACTACTGGCCAAATGTGG
GCCCGCAGCAGCTACCTGGGGGCTGTGTTCAATCTCACCAGCGCTGACCATTTATATGTC
AACGTATCTGAGCTCTCTCTGGTCAATTTTGAGGAATCTAAGACATTTTTTGGCTTATAT
AAGCTCTGA

处理步骤

• 将文件夹下gb文件批量读取
• 将各个gb文件中的登录号和具体序列抽提出来，并写入fasta文件
• 将fasta文件进一步处理去掉换行符，使一个完整的序列中没有换行符
• 将所有处理好的fasta文件存入一个新建的子文件夹中

Python脚本代码如下：

#!/usr/bin/env python
# -*- encoding: utf-8 -*-
'''
@File    :   gb2fasta.py
@Time    :   2020/07/04 14:15:13
@Author  :   Ai
@Version :   1.0
@Contact :   aqy0716@163.com
@License :   (C)Copyright 2020 SCAU
@Desc    :   将gb文件转换为fasta文件，同时转成无换行符，最后存入新的子文件夹中
'''
# here put the import lib
import os
import shutil
def gb2fasta(path):
    # 从gb文件中抽取登录号和具体序列信息存入同名fasta文件
    # 读取文件夹相关信息
    for root,dirs,files in os.walk(path):
        for file in files:
            # 打印文件所属目录
            print(root + '\\' + file)
            # 获取文件路径
            path_gb = os.path.join(root,file)
            flag=0
            if path_gb[-2:] == 'gb':
                # 打开新建fasta文件准备写入
                fasta = open (path_gb[:-2]+'fasta','w')
                # 打开gb文件，准备读取序列信息并写入fasta文件
                with open (path_gb,'r') as f:
                    # 逐行扫描
                    for line in f:
                        # 如果是ACCESSION行，则写入fasta文件作为序列标题
                        if line[0:9] =='ACCESSION':
                            fasta.writelines('>'+line.split()[1]+'\n')
                        # 如果是ORGIN行，代表是序列
                        elif line[0:6] =='ORIGIN':
                            flag = 1
                        elif flag == 1:
                            #通过空格符（空格 换行 制表）对字符串进行切片
                            s = line.split()
                            #非空切片字符打印
                            if s != []:
                                #print(s)
                                #去掉列表首个元素（数字序号）后，连接所有元素即为完整序列按行写入fasta文件
                                seq=''.join(s[1:])
                                fasta.writelines(seq.upper()+'\n')
                fasta.close()
def multi2single(path):
    #此函数功能为： 将多行序列转换为单行序列（即去掉换行符），成为标准fasta文件
    for root,dirs,files in os.walk(path):
        for file in files:
            path_full = os.path.join(root,file)
            # 有fasta 且不为 single.fasta，才进行单行转换，否则会重复创建文件夹
            a = path_full[-5:] == 'fasta'
            if path_full[-12:] == 'single.fasta':
                b = True
            else:
                b = False
            b = bool(1-b)
            if a & b :
                fr=open(path_full, 'r')
                fw=open(path_full[:-6]+'_single.fasta', 'w')
                seq={}
                for line in fr:
                    if line.startswith('>'):    #判断字符串是否以‘>开始'
                        name=line.split()[0]    #以空格为分隔符，并取序列为0的项。
                        seq[name]=''
                    else:
                        seq[name]+=line.replace('\n', '')
                fr.close()
                for i in seq.keys():
                    fw.write(i)
                    fw.write('\n')
                    fw.write(seq[i])
                    fw.write('\n')
                fw.close()
def copy2subdir(path):
    # 将生成的_single.fasta文件存入新的子文件夹.\singl_fasta\中
    root1 = path
    for root,dirs,files in os.walk(path):
        subdir = os.path.join(root1,'singl_fasta\\')
        for file in files:
            oldfile = os.path.join(root1,str(file))
            newfile = os.path.join(root1,'singl_fasta\\',str(file))
            if not os.path.exists(subdir):
                os.makedirs(subdir)
                print("目录创建成功！")
            if oldfile[-12:] == 'single.fasta':
                if not os.path.exists(newfile):
                    shutil.copyfile(oldfile , newfile)
    print("\n你的fasta文件保存在: " +  subdir  + "文件夹下\n")
if __name__ == "__main__":
    path = input("请输入路径") #此处输入 D:\docu\gb2fasta
    gb2fasta(path)
    multi2single(path)
    copy2subdir(path)

运行情况

PS D:\vscode_python_magic> & d:/ruanjiancangku/python_projectkotin/venv/Scripts/python.exe d:/vscode_python_magic/实验室-magic/序列处理/gb2fasta.py
D:\docu\gb2fasta\1-FASLG-swine-849bp-NM_213806.fasta
D:\docu\gb2fasta\1-FASLG-swine-849bp-NM_213806.gb
['1', 'atgcagcagc', 'ccttcaatta', 'cccatacccc', 'caaatcttct', 'gggtggacag', 'cagtgctacc']
['61', 'tctccctggg', 'cctccccagg', 'ctcagtcttc', 'ccctgtccag', 'cttctgtgcc', 'aggaaggcca']
['121', 'gggcaaagga', 'ggccaccacc', 'accaccgccg', 'ccaccgccac', 'caccaccaac', 'actcctgcca']
['181', 'tcaagaccgc', 'tgcctccact', 'gccaccgcca', 'tctctgaaga', 'agaagaggga', 'ccacaatgca']
['241', 'ggcctgtgtc', 'tccttgtgat', 'gttcttcatg', 'gttctggtgg', 'ccctggttgg', 'attggggctg']
['301', 'gggatgtttc', 'agctcttcca', 'cctacagaag', 'gagctgactg', 'aactcagaga', 'gtctgccagc']
['361', 'caaaggcata', 'cagaatcatc', 'tttggagaag', 'caaataggtc', 'accccaatct', 'accctctgag']
['421', 'aaaaaggagc', 'tgagaaaagt', 'ggcccactta', 'acaggcaagc', 'ctaactcaag', 'atccatccct']
['481', 'ctggaatggg', 'aagacaccta', 'tggaattgcc', 'ttggtctctg', 'gggtgaagta', 'tatgaagggc']
['541', 'agccttgtga', 'tcaatgacac', 'tgggctgtat', 'tttgtgtatt', 'ccaaagtgta', 'cttccggggt']
['601', 'cagtactgca', 'acaaccagcc', 'cctgagtcac', 'aaggtataca', 'caaggaactc', 'taggtatccc']
['661', 'caggacctgg', 'tgctgatgga', 'gggaaagatg', 'atgaactatt', 'gcactactgg', 'ccaaatgtgg']
['721', 'gcccgcagca', 'gctacctggg', 'ggctgtgttc', 'aatctcacca', 'gcgctgacca', 'tttatatgtc']
['781', 'aacgtatctg', 'agctctctct', 'ggtcaatttt', 'gaggaatcta', 'agacattttt', 'tggcttatat']
['841', 'aagctctga']
['//']
D:\docu\gb2fasta\2-LTA-swine-1584bp-NM_214453.fasta
D:\docu\gb2fasta\2-LTA-swine-1584bp-NM_214453.gb
['1', 'agaaaggggc', 'ccacaggggt', 'cccgcacagc', 'aggtgagact', 'ctcccacccc', 'atctcctagg']
['61', 'gctgtccggg', 'tgctggactc', 'ccccctcact', 'tcggtccctc', 'cgcccgctcc', 'ctggccttcc']
['121', 'tgcccctcct', 'gcatcttcac', 'cccggcctgg', 'gccttggtgg', 'gtttggtttt', 'ggtttgttct']
['181', 'ctctgattct', 'ttatctgtca', 'ggctctttct', 'agctctcaca', 'cactctgatc', 'cctctctgtt']
['241', 'cccttcccat', 'ctctgtttct', 'ctctgggtct', 'ccccctgctc', 'acctcgggat', 'ttccctgagt']
['301', 'gcctctggtc', 'cccttctctg', 'tctggcgccc', 'cgtctcttgt', 'ctctcggggt', 'ggctgtctcc']
['361', 'gagggcagga', 'ggccttcttc', 'cgcaggtgcc', 'ccgccccgct', 'cactgtctct', 'ctccccccac']
['421', 'aggttttccc', 'catgacacca', 'cctggacgcc', 'tctacctccg', 'gagggtgtgc', 'agcaccccca']
['481', 'tcctcctcct', 'cctggggctg', 'ctgctggccc', 'tgccgcccga', 'ggcccagggg', 'ctccctggcg']
['541', 'tcggcctccc', 'accctcagct', 'gcacagcctg', 'cccatcagca', 'ccccccaaag', 'cacttggcca']
['601', 'gaggcaccct', 'caaacctgcc', 'gctcacctcg', 'ttggagaccc', 'cagcaccccg', 'gactcactgc']
['661', 'gctggagagc', 'gaacacggat', 'cgtgccttcc', 'tccgccatgg', 'cttcttgctg', 'agcaacaact']
['721', 'ccctgctggt', 'ccccaccagt', 'ggcctctact', 'ttgtctactc', 'ccaggtcgtc', 'ttctccgggg']
['781', 'aaggctgctt', 'ccccaaggcc', 'acccccaccc', 'ctctctacct', 'ggcccacgag', 'gtccagctct']
['841', 'tctcctccca', 'gtaccccttc', 'cacgtgccgc', 'tcctcagcgc', 'tcagaagtcc', 'gtgtgccccg']
['901', 'ggccacaggg', 'accttgggtg', 'cgctctgtgt', 'accagggggc', 'tgtgttcctg', 'ctcacccagg']
['961', 'gagatcagct', 'gtccacacac', 'acagacggca', 'ccccccacct', 'gctcctcagc', 'cccagtagcg']
['1021', 'tcttctttgg', 'agccttcgct', 'ctatagaaga', 'atccagaaag', 'aaaaaaattg', 'gtttcaaggc']
['1081', 'cttctcccct', 'tttcacctcc', 'cttatgacca', 'cttcggaggt', 'caccgcgcct', 'ctcctctgac']
['1141', 'aatttccaac', 'agtctcatct', 'tcccccacgc', 'tcagcacctg', 'gagcttctgt', 'agaaggaatt']
['1201', 'ctaggcacct', 'cgggggaact', 'ggaaccaccc', 'cggatgctct', 'gctgaggatc', 'tgaatgcccg']
['1261', 'cctggagccc', 'ttcccctgtc', 'ctgcccgtct', 'aggggccctc', 'gtccaggacg', 'tggaagggaa']
['1321', 'gctgacccat', 'gagggacttt', 'gaacggatga', 'ccggagcggt', 'gtgggggggt', 'tatttatgaa']
['1381', 'ggggaaaatt', 'aaattattta', 'tttatggagg', 'atggagagaa', 'gggaatcaca', 'gagggatgtc']
['1441', 'agaagagtgt', 'gacacatgtg', 'cccaagagat', 'aaagtgacag', 'aaggcatggg', 'ctccagatga']
['1501', 'cccggccaga', 'gagggcaaag', 'tggctcagga', 'aggggctgct', 'tgactggagg', 'ctcatgagga']
['1561', 'gacggctgac', 'cctcgatgaa', 'accc']
['//']
D:\docu\gb2fasta\3-LTB-swine-950bp-NM_001185138.fasta
D:\docu\gb2fasta\3-LTB-swine-950bp-NM_001185138.gb
['1', 'tcggatgggg', 'gcaccggggc', 'tggagggccg', 'gggtaggagg', 'ccccagggga', 'agggatgcct']
['61', 'cctgctggcc', 'gtggcagggg', 'ccacttccct', 'ggtgaccctc', 'ctgctggccg', 'tgcctatcac']
['121', 'ggtcctggct', 'gtgctggcct', 'tggtgcccca', 'ggagcaggga', 'gaactggtaa', 'cagggaccgc']
['181', 'tgacccaggc', 'acccaggcgg', 'aggcccagca', 'gcgattggag', 'tccaaggaga', 'cgccagagga']
['241', 'ggaggcagaa', 'acagatctca', 'gccccaggct', 'cccagctgcc', 'cacctcattg', 'gcgcttggat']
['301', 'cacgggtcag', 'gggctaggct', 'gggaggcgaa', 'gaaagaagag', 'gcgtttctga', 'ggagcgggac']
['361', 'gcagttctct', 'ggcgcggagg', 'gcctggccct', 'cccgcaggac', 'ggcctctact', 'acctctactg']
['421', 'tcacgtcggc', 'taccggggcc', 'gggcacctcc', 'tcccggcggg', 'gaccccctgg', 'accgctcggt']
['481', 'cacgctgctc', 'agccggctgt', 'accgggcggg', 'gggcgcctac', 'ggaccgggga', 'ctcccgagct']
['541', 'gctgctggag', 'ggcgcggaga', 'ctgtgactcc', 'ggtcttggac', 'cccagtcgga', 'ggcacgagta']
['601', 'cgggcccctc', 'tggtacacga', 'gcgtggggtt', 'cggtggcctg', 'gtgcagctcc', 'ggaggggcga']
['661', 'gagggtgtac', 'gttaatatca', 'gtcaccccga', 'tatggtggat', 'tacaggagag', 'gaaagacctt']
['721', 'cttcggggcg', 'gtgatggtgg', 'gctgaggact', 'gtccgcggcc', 'cgagaggacc', 'actgcatggt']
['781', 'gggagtgtgt', 'cgatggatca', 'agcccagaca', 'cggggtccca', 'gacaccaggc', 'cagacaccat']
['841', 'ggccgtgggg', 'aaaatgcagg', 'agatcgtgtg', 'gaaaactgat', 'tttgagcctg', 'atgaaaataa']
['901', 'agaatgtaaa', 'agctttaatn', 'gctgcccatg', 'ccaaaaaaaa', 'aaaaaaaaaa']
['//']
D:\docu\gb2fasta\4-TNF-swine-1666bp-NM_214022.fasta
D:\docu\gb2fasta\4-TNF-swine-1666bp-NM_214022.gb
['1', 'cccagagtga', 'ggacaccagg', 'ggaccagcca', 'ggagagagac', 'aagccatctc', 'caggaccccc']
['61', 'tagaaataac', 'ctctcagaag', 'acacaccccc', 'gaacaggcag', 'ccggacgact', 'ctctccctct']
['121', 'cacacgctgc', 'cccggggcgc', 'caccatctcc', 'cagctggacc', 'tgagcccctc', 'tgaaaaagac']
['181', 'accatgagca', 'ctgagagcat', 'gatccgagac', 'gtggagctgg', 'cggaggaggc', 'gctcgccaag']
['241', 'aaggccgggg', 'gcccccaggg', 'ctccaggagg', 'tgcctgtgcc', 'tcagcctctt', 'ctccttcctc']
['301', 'ctggtcgcag', 'gagccaccac', 'gctcttctgc', 'ctactgcact', 'tcgaggttat', 'cggcccccag']
['361', 'aaggaagagt', 'ttccagctgg', 'ccccttgagc', 'atcaaccctc', 'tggcccaagg', 'actcagatca']
['421', 'tcgtctcaaa', 'cctcagataa', 'gcccgtcgcc', 'cacgttgtag', 'ccaatgtcaa', 'agccgaggga']
['481', 'cagctccaat', 'ggcagagtgg', 'gtatgccaat', 'gccctcctgg', 'ccaacggcgt', 'gaagctgaaa']
['541', 'gacaaccagc', 'tggtggtgcc', 'gacagatggg', 'ctgtacctca', 'tctactccca', 'ggtcctcttc']
['601', 'aggggccaag', 'gctgcccttc', 'caccaacgtt', 'ttcctcactc', 'acaccatcag', 'ccgcatcgcc']
['661', 'gtctcctacc', 'agaccaaggt', 'caacctcctc', 'tctgccatca', 'agagcccttg', 'ccagagggag']
['721', 'acccccgagg', 'gggccgaggc', 'caagccctgg', 'tacgaaccca', 'tctacctggg', 'aggggtcttc']
['781', 'cagctggaga', 'aggatgatcg', 'actcagtgcc', 'gagatcaacc', 'tgcccgacta', 'tctggacttt']
['841', 'gctgaatctg', 'ggcaggtcta', 'ttttgggatc', 'attgccctgt', 'gagggggcag', 'gacatccgtt']
['901', 'ccctcccctg', 'tccatccctt', 'tattatttta', 'ctccttcaga', 'ccccctcacg', 'tccttctggt']
['961', 'ttagaaagag', 'aatgaggggc', 'tggggactgg', 'gctccaagct', 'taaaacttta', 'aacaacaaca']
['1021', 'gcaacactta', 'gaaatcaggg', 'attcagggat', 'gtgtggcctg', 'gacaaccagg', 'cactgaccac']
['1081', 'caccaagaat', 'tggaactggg', 'gcttccagac', 'tcgctggggt', 'ccttgggttt', 'ggattcctgg']
['1141', 'atgcaacctg', 'ggacatctgg', 'aatgtggctg', 'ccagggaagc', 'ttgggttcca', 'atcggaatac']
['1201', 'ttcagaacat', 'tccttgagaa', 'gatttcacct', 'caatcttgat', 'gactttttag', 'gcttcccttt']
['1261', 'cttccaattt', 'tccagacttc', 'cctgggatgg', 'ggagcccagc', 'cccaaacccc', 'acaggccagc']
['1321', 'tccctcttat', 'ttatatttgc', 'acttggcatt', 'attatttatt', 'tatttattta', 'ttatttattt']
['1381', 'actagtgaat', 'gtatttattc', 'aggagggcga', 'ggtgtcctgg', 'gagacccagc', 'ataagggctg']
['1441', 'ccttggttca', 'gatgtgtttt', 'ctgtgaaaac', 'ggagctgaac', 'tgtaggttgc', 'tcccacctgg']
['1501', 'cctcctagcc', 'tctgtgcctc', 'cttttgctta', 'tgtttttaaa', 'aacaaatatt', 'tatctgatcg']
['1561', 'agttgtctaa', 'ataatgctga', 'tttggtgact', 'aacttgtcgc', 'tacatcgctg', 'aacctctgct']
['1621', 'ccccagggga', 'gttgtgtctg', 'taaccgccct', 'actggtcagt', 'ggcgag']
['//']
D:\docu\gb2fasta\5-TNFSF4-swine-549bp-NM_001025217.fasta
D:\docu\gb2fasta\5-TNFSF4-swine-549bp-NM_001025217.gb
['1', 'atggaagggg', 'tccaacccct', 'agatgaaaat', 'gtgggaaacg', 'caccaggacg', 'aagactcttg']
['61', 'aggaacaagc', 'tattgttggt', 'ggcctccgta', 'attcagggtc', 'tggggttgct', 'cctgtgtctc']
['121', 'acctacatct', 'gcctgcacct', 'ctatgctcag', 'gtgccatctc', 'agtaccctcc', 'aattcagagt']
['181', 'atcaaagtac', 'aatttaccaa', 'gtgtgaaaat', 'gataatggtt', 'tcatcatcac', 'accctcaagc']
['241', 'aaggatggaa', 'ccatgaaagt', 'gcaaaacaac', 'tcaatcatca', 'tcaactgtga', 'tgggttctat']
['301', 'ctcatctccc', 'tgaagggtta', 'cttttctcag', 'gagctcagcc', 'tcatgcttca', 'gtaccggaag']
['361', 'ggtcggaaac', 'ctctcttctc', 'cctgaacaag', 'gtcaagtctg', 'tggactctgt', 'cacagtagcc']
['421', 'gatctggctt', 'tcaaggacaa', 'ggtcttcctg', 'aacgtgacca', 'ctcatagtgc', 'ctcctgtgaa']
['481', 'gacattcagg', 'tgaatggtgg', 'ggaattgatt', 'ctcattcatc', 'aaaatcctgg', 'tggattctgt']
['541', 'gtctactga']
['//']
D:\docu\gb2fasta\6-TNFSF10-swine-1696bp-NM_001024696.fasta
D:\docu\gb2fasta\6-TNFSF10-swine-1696bp-NM_001024696.gb
['1', 'agcagtcaga', 'ccctgcctgg', 'accatggcgg', 'tgatgcagac', 'tccaggaggc', 'cccagccccg']
['61', 'ggcagacctg', 'tgtgttgatc', 'ctgatcttca', 'cagtgctcct', 'gcaagccctc', 'tgtgtggcct']
['121', 'tgacttacgt', 'gtacttcacc', 'aatgaactga', 'aacagatgca', 'ggacaagtac', 'tccaaaagcg']
['181', 'gtatagcttg', 'cttcttaaag', 'gaagatgaca', 'gtttctggga', 'tcccaccgat', 'gacgagagaa']
['241', 'tgctcagccc', 'ctgctggcag', 'gtgaagtggc', 'agctacgtca', 'gtttgtgaga', 'aagatgattt']
['301', 'tgagaaccta', 'tgaggaaacc', 'atttctacag', 'tttcagaaaa', 'gcaacaaggc', 'attcctcacc']
['361', 'tagaaagaga', 'aaaaggtcca', 'cagagagtgg', 'ctgctcacat', 'aactggaacc', 'agtaggaaaa']
['421', 'gaagcacatt', 'tccatctcta', 'agctccaaat', 'atgaaaaagc', 'tttgggccag', 'aaaataaact']
['481', 'cctgggaatc', 'atcaagaaaa', 'ggacattcat', 'tcttgaataa', 'ttttcacttg', 'aggaatggag']
['541', 'agctggttat', 'ccatcaaaca', 'gggttttact', 'acatctattc', 'ccaaacatac', 'tttcgatttc']
['601', 'aggaacctga', 'ggaaattttg', 'ggaacggttt', 'ctacagaagg', 'gaacagaaag', 'aaaaacaggc']
['661', 'aaatgataca', 'gtatatttac', 'aaatggacaa', 'gctatcctga', 'ccctatactg', 'ctgatgaaaa']
['721', 'gtgctagaaa', 'tagttgttgg', 'tctaaagatt', 'cagaatatgg', 'actctattcc', 'atctatcaag']
['781', 'gtggaatatt', 'tgagcttaag', 'gaagatgacc', 'gaatttttgt', 'ctctgttact', 'aatgagcaac']
['841', 'tgattgacat', 'ggaccaagaa', 'gccagttttt', 'tcggggcctt', 'tttaattggc', 'taaatgatct']
['901', 'gcagggaaaa', 'aaaccatgcc', 'ccagagtgac', 'tattcagagt', 'cgtatactgt', 'gaaaatattc']
['961', 'cagcagagcc', 'aataggttaa', 'ggcagcctga', 'gcaaagaggc', 'ctcaacccaa', 'aggctcaaca']
['1021', 'acacaagctt', 'tttggaaagt', 'gaaaagtgac', 'caattccttc', 'caggaaaatg', 'aaactgccaa']
['1081', 'gagaccttgt', 'ggagctctgc', 'ctgatgtcat', 'tttgctagta', 'aacatctaga', 'agatactctg']
['1141', 'tctccaaatt', 'tgtgtaacaa', 'ttaacacctc', 'ctgcctttat', 'catctaatcc', 'tgtgaagatt']
['1201', 'ctagaagaaa', 'gagtagtgat', 'ccatctcagg', 'tgggaataag', 'ggacaacatt', 'cccaaaacta']
['1261', 'aagagaaaag', 'ggcagcactg', 'aaaggtcaca', 'gtcaatatat', 'gcagtttcag', 'tacaaacata']
['1321', 'acaaattaaa', 'gctacgttta', 'gtggacaagg', 'agctacttct', 'gaatggtttg', 'tgcttttctc']
['1381', 'tactaaaaat', 'caggctggcc', 'aaaagcactc', 'agggtatttt', 'tgataaagga', 'ctctaaaata']
['1441', 'agtgataaag', 'tatggcgata', 'cctcagaaaa', 'ctaaatacag', 'aactaccaca', 'tgacccagca']
['1501', 'atcccactcc', 'tgtgcatata', 'tctggacaaa', 'actttccttg', 'aaaaagatac', 'attcatctct']
['1561', 'atgctcattg', 'cagcactatt', 'cacagtagcc', 'aagacatgga', 'aacacctata', 'tgtctatgaa']
['1621', 'tggatgaata', 'gattaagaag', 'gtgtgttatg', 'tatacatant', 'ggaatactgg', 'gaagccataa']
['1681', 'aaaggacaaa', 'gaggcc']
['//']
D:\docu\gb2fasta\7-TNFSF13b-swine-1013bp-NM_001097498.fasta
D:\docu\gb2fasta\7-TNFSF13b-swine-1013bp-NM_001097498.gb
['1', 'tctaggaggg', 'aaatggatga', 'ctccacgggg', 'gagcagtcac', 'gcctttcttg', 'ccttagcacg']
['61', 'agagaagaaa', 'tgaaactgaa', 'ggagacggtc', 'cccatcctcc', 'cccagaagga', 'aagcccctct']
['121', 'gtccgcatct', 'ccaaagatgg', 'gaagctgctg', 'gtcgtgaccc', 'tgctgctggc', 'cctgctgtcc']
['181', 'tgctgcctca', 'cggggatctt', 'tgcaccacca', 'gctccaaggg', 'agagcagctc', 'cattcaaagc']
['241', 'aacaggagta', 'agcgcgccgc', 'gcaggatgcg', 'gaggagacag', 'tcactcagga', 'ctgcttgcaa']
['301', 'ttgattgcag', 'acagtgacat', 'gcctactata', 'cgaaaaggag', 'cttatacatt', 'tgttccatgg']
['361', 'cttctcagct', 'ttaaaagagg', 'aagagcccta', 'gaagaaaaag', 'aaaataaaat', 'cgtggtcaaa']
['421', 'gaaacgggtt', 'acttttttat', 'atacggtcag', 'gttttataca', 'ccgataacac', 'ctttgccatg']
['481', 'gggcatctca', 'tacagaggaa', 'gaaagtccat', 'gtctttgggg', 'atgaactgag', 'tctggtgact']
['541', 'ttgttccgat', 'gtattcaaaa', 'tatgcctgaa', 'acactaccca', 'ataattcctg', 'ttattcagct']
['601', 'ggcattgcaa', 'agctggagga', 'aggagatgaa', 'ctccaactgg', 'caataccacg', 'tgaagacgct']
['661', 'aaaatatcac', 'gggatggaga', 'cggcacattt', 'tttggtgcat', 'tgaaacttct', 'gtgacctact']
['721', 'tacaccttgt', 'ttgtggctct', 'tgccctccct', 'ccctctgtac', 'ctctaaagag', 'aaaacactta']
['781', 'actggaaata', 'ccaaaagggg', 'aaaaaaaagt', 'agttaccata', 'gccttttctg', 'tgagctgttt']
['841', 'gttttggttt', 'gctgaaacta', 'gaccaaaaca', 'ggaaatttaa', 'cagacaacca', 'cagccaaagg']
['901', 'gtatcatgtg', 'aattacaaga', 'aatagagccc', 'atttaagaaa', 'aaatagaatt', 'agaaagactt']
['961', 'ttcactgtaa', 'tgccatgttg', 'aacagcttag', 'tcatagcttc', 'ttgtcttgga', 'gga']
['//']
D:\docu\gb2fasta\8-TNFSF18-swine-5005bp-XM_005667782.fasta
D:\docu\gb2fasta\8-TNFSF18-swine-5005bp-XM_005667782.gb
['1', 'gcattccatt', 'taacaaatga', 'aaaggctaag', 'gcataaagaa', 'ccaggagaga', 'gaaccggaga']
['61', 'tttcctcaat', 'tttagtgtag', 'taaatcaaca', 'gttttagtgc', 'taggaagttt', 'tttggaatag']
['121', 'agtgtaaact', 'cagatggtag', 'gacagggtgc', 'atgaaaatat', 'ccttttctat', 'gataacttta']
['181', 'tttctgtctg', 'atgtcagcat', 'tgaaatttca', 'gagtattaaa', 'atggtgaggt', 'atgaaaaaac']
['241', 'taagcttgtt', 'gttattgaca', 'tatttttaaa', 'aataaaattc', 'tagtaataca', 'ttactgtttt']
['301', 'ctagagatta', 'tctcagaatg', 'gacacattgt', 'ataccctagc', 'aattgatgaa', 'aatatttttc']
['361', 'ccaaaccatg', 'aaccccagca', 'tccttagctc', 'cctgacctac', 'tgcctcccac', 'aaacatgata']
['421', 'tttggagtat', 'gatagacctt', 'catcttgaat', 'ttcattcttt', 'ttcttacaaa', 'agtaattttc']
['481', 'ttatctggaa', 'ataatttgta', 'atgttgaata', 'gttccatagt', 'tcctcttgct', 'tcagaaaata']
['541', 'tttatttttc', 'ctcttaactt', 'cccttgttgg', 'gttttttttt', 'tttaaatcat', 'ctgtgtttgt']
['601', 'gttggcttct', 'agccctcagt', 'tccagcacct', 'ttggtctggt', 'gccaaatgtt', 'agtcagcact']
['661', 'taggctaaaa', 'gtatcgtttt', 'ccaacaccca', 'gatcagaagg', 'aaaactccgc', 'ctcttacacc']
['721', 'cactacttag', 'tgctatacta', 'caaaactgac', 'tagttgaatc', 'atgtgctcat', 'tacttctgaa']
['781', 'tttctgcttt', 'tcacaactct', 'cattcctgca', 'gagaatgagt', 'ttgagccaca', 'tggagaatat']
['841', 'gcctttaagc', 'cattcaagtc', 'ctcacgcagc', 'acagagacca', 'tcctggaagc', 'aatggctact']
['901', 'ctactcaaca', 'atagttattt', 'tgctattact', 'ttgctccttc', 'agtgcactaa', 'tcttaacttt']
['961', 'tctcccactc', 'aagacctcca', 'acaggccatg', 'tgtagcgaag', 'tttggaccat', 'taccttcaaa']
['1021', 'atggcaaatg', 'ccatctcctg', 'agccttcttg', 'tgtgaataag', 'acagatgatt', 'ggaggctgaa']
['1081', 'gatacttcag', 'aatggcttgt', 'atttaattta', 'tggccaagtg', 'gctcccaaca', 'cagcttacaa']
['1141', 'ggggcaagct', 'ccttttgagg', 'tgttgctacg', 'taggaatgaa', 'gaccccatac', 'aatctctaac']
['1201', 'gaacaattct', 'acagtccaga', 'atgtaggagg', 'ggcttatgaa', 'tttcatgctg', 'gagatgtaat']
['1261', 'agacttgata', 'ttcaatgctg', 'aacatcaggt', 'tctaaaaaat', 'aatacatact', 'gggggatctt']
['1321', 'tctgctagca', 'aatccccaat', 'tcatctccta', 'gagactcagt', 'taggtctcct', 'catcttcagc']
['1381', 'acatgcagag', 'atgccagtgc', 'ataggatgga', 'gaaggaagat', 'tttcaacaca', 'tacagttcat']
['1441', 'ctgggtatac', 'aaatcaacat', 'gaacagatct', 'cctctgcatg', 'tgaagcttca', 'tttctcctgc']
['1501', 'ttattgaatg', 'agactcagaa', 'agcactgaag', 'acatttggtt', 'acccctgatg', 'ttgggtcagc']
['1561', 'aaagacactt', 'tactagttca', 'tgataaaatg', 'aaaatgggtg', 'gctggaagac', 'aaaatctttt']
['1621', 'caaagtgtct', 'gtctaatcct', 'tgaacccctg', 'agtggaaaaa', 'tgaggtctat', 'tcccataata']
['1681', 'gccttatata', 'gcatgcaaaa', 'aaagaccagg', 'gcagtagcct', 'ggtcttgttc', 'ttatattctt']
['1741', 'ggactgtgga', 'ctgtttcaat', 'tcattcttcc', 'catattctca', 'tcttaggaga', 'cactcttaat']
['1801', 'aaaatgtagt', 'cagagtgggt', 'gtgtggccag', 'caacactcca', 'ttttggagtt', 'gatgagatta']
['1861', 'ggggatagag', 'aacactctta', 'ggaaatattg', 'ggacagaatt', 'tcagttggca', 'ttgaaatgga']
['1921', 'atgcacttta', 'ttcgggaatt', 'tcacttgatt', 'tcatcatcaa', 'gtgcagggtg', 'ctctataaaa']
['1981', 'cctgctggtc', 'aaaaggctag', 'ctttcaatct', 'tcacatagca', 'gttcatgaga', 'atttactggt']
['2041', 'gtatgtatct', 'aaccatgtca', 'atgacaaaga', 'gtaatcatta', 'gtagtaagat', 'ctaacccccc']
['2101', 'aaattggtat', 'taccagtact', 'gtactttgca', 'actgtgcaga', 'gccagctaaa', 'aatatgaaat']
['2161', 'cattacatga', 'caaagcactt', 'tcatatacca', 'catggcaact', 'cgatagattt', 'aatggggcag']
['2221', 'atatttttgg', 'cacaatttta', 'actatgagaa', 'tacagaggca', 'gatggaatag', 'aggtaacttg']
['2281', 'gttcagttca', 'tagaactagt', 'aattaacagg', 'cacctgggct', 'tcccactgta', 'ttatactata']
['2341', 'ttagctttac', 'gattggtatt', 'tctgctatca', 'tgttagaagc', 'ctataaactt', 'taacagattt']
['2401', 'aaaattttca', 'gacagtatat', 'tcccttttag', 'tccaacagca', 'attttttcct', 'ttctcagcaa']
['2461', 'atttcttttc', 'ttttctttgc', 'ctggagcagg', 'gtacccaggg', 'tgttattcaa', 'gacttactac']
['2521', 'aacttaatct', 'ccttccttac', 'tttggtcaaa', 'tgtgttaact', 'tccaaaaata', 'atgaataata']
['2581', 'ctcaattcag', 'ggacagtctg', 'ttaaattttt', 'ggactctgca', 'aaattaacta', 'gctgcttatg']
['2641', 'ggttgttatt', 'aaaaggtatg', 'taggtaatgt', 'gattacatga', 'aaacccaatt', 'taaaatattt']
['2701', 'atggatattt', 'gtaaaaaatc', 'tacattatgt', 'taattaatag', 'tatcaccatt', 'aaaaactaat']
['2761', 'ttaagaatat', 'ttgtattgta', 'tgtaagaaaa', 'actgcttgga', 'agcagactaa', 'gcctgaggcc']
['2821', 'aagatgcctc', 'atagtatgtc', 'tttttttttt', 'tttttttaaa', 'tacatctgct', 'gagcagctgt']
['2881', 'agggacaaag', 'actggggtac', 'ctggttcctc', 'ttgtatttgt', 'gtatcatctc', 'aggaaattaa']
['2941', 'agttacataa', 'catacatata', 'tttatggaaa', 'cgtggtattg', 'atgttaactt', 'ataagcagta']
['3001', 'gtgtgctgga', 'gtgggctagc', 'actagctcag', 'gagagctgtt', 'aaatttttat', 'taattgtgta']
['3061', 'gtctggttat', 'taaatcatta', 'tccttgaaat', 'tggccatggt', 'aggacaattt', 'ataccatgtg']
['3121', 'aattagcaaa', 'tgctacaaat', 'cagggctttt', 'ctttttggaa', 'agcccatgca', 'ccagcacacc']
['3181', 'actgtttata', 'aaactcttct', 'taatgactcc', 'tctcagcccc', 'tgcctcagta', 'ttacaacagt']
['3241', 'caaggcaggc', 'aaggaaagtg', 'tcttactctc', 'agcaaaagcc', 'ccacagataa', 'atcatttctc']
['3301', 'agggcaggtg', 'gaggaatcta', 'cagctgtaac', 'cagatagata', 'gctaccaaca', 'tatgaccttt']
['3361', 'gaatttccct', 'agtgttgaaa', 'tttcaggctt', 'tgttttcaat', 'gtatactctg', 'ttcccttgtt']
['3421', 'tcttcaaaac', 'agtgtttata', 'ttttaaactg', 'acaataaaat', 'gtttgtacat', 'gggctgtagc']
['3481', 'tgatttatct', 'atgggttatc', 'tgacaagagt', 'tagtgatcta', 'gagaatgtgc', 'atcagagaaa']
['3541', 'tccttgtcca', 'tatgaaacac', 'tgctgagaga', 'gtgagaggca', 'aaagtcagag', 'ctgggggttt']
['3601', 'tgtcccaatt', 'tctgatgggt', 'taggcaaata', 'atttcactag', 'ctgactttca', 'cccacctcct']
['3661', 'caactctacc', 'ccacccatcc', 'accctcacca', 'tcaaagccat', 'catcaaccct', 'ctcatactaa']
['3721', 'ttgtttttac', 'cttctatttt', 'gcctcatcaa', 'agctgacctg', 'gagtgctttt', 'tatcactgtg']
['3781', 'ggttaagtct', 'ctacttctga', 'agatgacagt', 'taattgatca', 'cagtcctgtg', 'tatttgatgg']
['3841', 'aacagataaa', 'ctccttagat', 'gcattatatc', 'tttgcaactt', 'ggagataaaa', 'ttcctgtctt']
['3901', 'tgtgtgttct', 'tcctcaaaga', 'agcaattttc', 'attggctggg', 'ggtgctagta', 'cgttttttat']
['3961', 'tagtgagaag', 'gagaagcgaa', 'aacccaattg', 'acttatgggc', 'tagtggtctg', 'aggcttacct']
['4021', 'tcaagcagag', 'tttttcaaag', 'tgctatctat', 'ggactctaca', 'ttagatttgc', 'ccagtatact']
['4081', 'tggccaaaaa', 'ccaaagaaaa', 'accccaaaga', 'taccttgacc', 'tcattcatag', 'tccactaaat']
['4141', 'aaatgtttct', 'aggagtgagg', 'tctaggaatc', 'aaatttgtaa', 'tgagctctga', 'cataggttat']
['4201', 'tcttatacta', 'cagtttaaaa', 'accactgctt', 'tatggaatgc', 'aattttgaat', 'ctcagcagaa']
['4261', 'tgtttctcca', 'aatatttaaa', 'aacagtcaaa', 'gcttataaac', 'caatgaagtt', 'aaaaagcaaa']
['4321', 'gcaaaaagca', 'aaaccaaaat', 'tggtattcat', 'gagacttacc', 'ataagccatc', 'tctcatcaca']
['4381', 'aacaatggcc', 'ttctatcctc', 'tatttcagtt', 'tgaaaaaaag', 'gtcaggctct', 'tcactttcca']
['4441', 'taatctaatg', 'gcaactttgc', 'cagttgaatg', 'ctggcaatcc', 'atagcatatg', 'atccttgtca']
['4501', 'taatcagtac', 'tccattatct', 'acctgtatgc', 'tgtcaactct', 'caaatactta', 'tctctagcca']
['4561', 'aagttgttct', 'cctttaagtc', 'ttataactcc', 'acctgtctgc', 'acagtaattt', 'cacattggac']
['4621', 'atacaatgga', 'catctcaaat', 'tcaacttacc', 'ccaaatgaat', 'cttttaaggt', 'ttcaactaaa']
['4681', 'gcagcttttt', 'tcgaatccct', 'cctcatttca', 'attgtatctc', 'atgagccaaa', 'aacctctgaa']
['4741', 'tcatggagtc', 'attcctgact', 'tctatcttta', 'atctgtttaa', 'tcttaggaaa', 'actattatct']
['4801', 'ttactttcag', 'agaatctgac', 'ctctttttgg', 'ctatggctct', 'tgtctttgga', 'ccaatatttt']
['4861', 'tttcagtatc', 'ttcataactt', 'attacagtat', 'ctacatactt', 'attacattca', 'ggatttttgg']
['4921', 'actagaaact', 'gaattttatc', 'aaagtcttag', 'agtttatata', 'tctctcatat', 'atgaaaaacc']
['4981', 'acagctaaca', 'tcattcacaa', 'tggtg']
['//']
D:\docu\gb2fasta\gb2fasta.py
目录创建成功！
你的fasta文件保存在: D:\docu\gb2fasta\singl_fasta\文件夹下

程序运行前 D:\docu\gb2fasta\ 文件夹下 文件

运行前

程序运行后 D:\docu\gb2fasta\ 文件夹下 文件

运行后

程序运行后 D:\docu\gb2fasta\ singl_fasta\ 文件夹下 文件

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